Topics which the guide covers include heating systems, lighting, IT equipment and energy management.
Hugh Jones, director of solutions at the Carbon Trust, said: "Many firms tried to cut costs to the bone at the end of last year. But many could save further, significant amounts of cash, without affecting their service or production levels, by tackling energy efficiency."
The guide is aimed at firms which spend £50,000 or less on energy bills throughout the year.
A survey by the Confederation of British Industry found that over one-third of businesses had switched gas or electricity supplier in the past year in a bid to reduce their energy payments.
Click here to run an energy price comparison, and see if you could be paying less for your gas and electricity.